Protective effect of Satureja montana extract against cyclophosphamide-induced testicular damage in rats

Cyclophosphamide, a chemotherapeutic compound, causes testicular toxicity and affects fertility by inducing oxidative stress and apoptosis in testicular cells. This study investigates for the first time the effect of Satureja montana (winter sedge) on protecting the testes from cyclophosphamide-induced damage in rats.

Method: Extract of Satureja montana was administered orally to rats for seven days before and after injection of cyclophosphamide. Testicular weight, testosterone production and enzyme activities were measured, and oxidative stress and apoptosis levels were evaluated.

Results: The study showed that Satureja montana extract significantly protected the testes from the harmful effects of cyclophosphamide. The extract reduced lipid peroxidation, improved antioxidant capacity, and counteracted apoptosis by upregulating anti-apoptotic factors such as Bcl-2 and PPAR-γ, as well as normalizing Akt1 protein levels. Histological analyzes confirmed that the plant preserved testicular tissue integrity and improved testicular health after injury.

Discussion: Oxidative stress is a key factor in male infertility and is associated with testicular damage. Satureja montana was shown to have strong antioxidative and anti-apoptotic effects, partly by regulating important proteins such as PPAR-γ and Akt1, which helped to restore normal testicular functions .

Conclusion: Satureja montana may be a promising, natural treatment method to counteract chemotherapy-related testicular damage. This study demonstrates its potential as a safe and effective way to protect the testes from cyclophosphamide-induced damage via antioxidant and anti-apoptosis mechanisms.

Question 1:
What is testosterone?
Answer:
Testosterone is a hormone that is mainly produced in the testicles of men. It regulates the development of male characteristics such as muscle growth, sex drive and sperm production. In women, it is produced in smaller amounts in the ovaries.

Question 2:
How do the testicles affect testosterone production?
Answer:
The testicles produce testosterone when stimulated by luteinizing hormone (LH), which comes from the pituitary gland. This hormone activates Leydig cells in the testes, which are responsible for testosterone production.

Question 3:
What symptoms occur with low testosterone levels?
Answer:
Common symptoms of low testosterone levels include fatigue, decreased muscle mass, decreased sex drive, erectile dysfunction, and mood swings.

Question 4:
How does testosterone affect fertility?
Answer:
Testosterone is essential for sperm production, and a lack of testosterone can reduce fertility by reducing the number of sperm produced.

Question 5:
Can testicular damage affect testosterone levels?
Answer:
Yes, damage to the testicles can lead to a reduced ability to produce testosterone, which can cause hormonal imbalance and problems with sexual function and fertility.

Question 6:
How to increase testosterone levels naturally?
Answer:
Exercise, healthy diet, adequate sleep and reduced stress can increase testosterone levels naturally. Supplements such as vitamin D and zinc can also help.

Question 7:
What is Satureja montana and how can it protect the testicles?
Answer:
Satureja montana is a plant that has antioxidant and anti-apoptotic properties. It has been shown to protect the testes against damage caused by cyclophosphamide, a chemotherapy compound, by reducing oxidative stress and promoting cell survival.

Question 8:
How does Satureja montana work to protect the testicles?
Answer:
Satureja montana protects the testicles by reducing oxidative stress and apoptosis (programmed cell death). It does this by regulating proteins such as Bcl-2 and PPAR-γ, which play an important role in cell survival and antioxidant defense.

Källa: Abd El Tawab, A. M., Shahin, N. N., AbdelMohsen, M. M. (2014). Protective effect of Satureja montana extract on cyclophosphamide-induced testicular injury in rats. Chemico-Biological Interactions, 224, 196-205. DOI: 10.1016/j.cbi.2014.11.001.